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KMID : 1098420050130060273
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 6 p.273 ~ p.277
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Efficient Procedures for Direct Shoots Regeneration from Leaf Explants of Rehmania glutinosa Lib.
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Hwang Sung-Jin
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Abstract
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Adventitious shoots were directly induced from leaf explants of R. glutinosa, an important medicinal plant. Proliferating shoot cultures were obtained by culturing leaf discs on Murashige and Skoog(MS) medium alone or combination with auxins and cytokinins. MS medium supplemented with 1 mg/{ell};BA;and;2;mg/{ell} IAA was the most effective, providing high shoot bud formation frequency without formation of intervening callus. The effect of leaf age on adventitious shoot formation was also investigated. The maximum shoot bud production (93.4%) was achived using 3rd leaf from apex of 6 weeks old plantlets after seed germination. Plantlet were rooted on an half-strength MS (1/2MS) medium containing 0.1 mg/{ell};IBA. This prtocol is useful for clonal propagation and Agrobacterium-mediated transforamtion in R. glutinosa.
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KEYWORD
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leaf discs, plantlet regneration, Rehmannia glutinosa
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